Sometimes, we get this error message after publishing the solution in IIS. This error is quite common.
Exact Error
Error message 401.2.: Unauthorized: Logon failed due to server configuration. Verify that you have permission to view this directory or page based on the credentials you supplied and the authentication methods enabled on the Web server. Contact the Web server’s administrator for additional assistance
This particular error happens mostly because of the authentication setting in IIS.
Solution
Open the IIS.
Select the site you are facing the issue.
Select Authentication Setting as shown,
Based on your application requirement, you can update authentication options.
In my case, the application needs to set windows authentication, therefore, I set it to enabled and Anonymous Authentication Disabled.