When host to IIS,the styles in CSS can't be applied

Question

Hi,

   I develop a web application in ASP.NET with C#.In debug mode it runs perfectly.The CSS styles are applied correctly.But when i publish the project and host it to IIS,the application runs without CSS styles. That looks an empty environment in designing.I can't understand what's the problem behind this.Whether in IIS version or in links to CSS files,where is the problem i confused about that.Can you tell about these problem guys..