How to connect web server to application server

Question

Dear All,
 
I have facing problem to connect the web server and application server in window IIS.
 
My client having three sever - App Server, Web Server, DB Server.
 
Actually my web project in asp.net c#, When I published code the compiler create DLL for the business login.
 
Can I do that my all aspx file in web server and the Bin folder which contain the DLL keep at application server. Am i thinking write? Please suggest for right way.