How to Arrange Ascending Order in my C# DataGried view

Question

 Hi Friend,
  
                i am develop the hospital project in C#.net[3.5 Framework].i show the non occupied beds in DataGriedView. i have use

                                 dataGridView1.Sort(dataGridView1.Columns[0], ListSortDirection.Ascending);

above code.but DataGriedView data  not arrange in ascending order.please help me... very urgent