Database connection error

Question

I have a asp.net application develop with visual studio 2008. my database is SQL server 2000. and this is pc2. visual studio is pc1 and also published in pc1. when i open the application as debug mood I found no error and I can work with pc2 database. but when i have published the application at pc1 then database is not connected. 
 
I could not understand what is the problem. Please help.