Can i add layout designed related css Or JS in Bundlconfig?

I am adding all layout design related .css or .JS file in "bundleconfig.cs".... or creating new Jquery function  file(.js)....it also added in 'Bundleconfig.cs' but not in work........can i add config file to layout design related......

 

....................My below code...................
public class BundleConfig
{
// For more information on bundling, visit http://go.microsoft.com/fwlink/?LinkId=301862
public static void RegisterBundles(BundleCollection bundles)
{
bundles.Add(new ScriptBundle("~/bundles/jquery").Include(
"~/Scripts/jquery-{version}.js",
"~/Scripts/AllJqueryFunction.js"
));

bundles.Add(new ScriptBundle("~/bundles/jqueryval").Include(
"~/Scripts/jquery.validate*"));

bundles.Add(new ScriptBundle("~/bundles/modernizr").Include(
"~/Scripts/modernizr-*"));

bundles.Add(new ScriptBundle("~/bundles/bootstrap").Include(
"~/Scripts/bootstrap.js",
"~/Scripts/respond.js"
));

bundles.Add(new StyleBundle("~/Content/css").Include(
"~/Content/bootstrap.css",
"~/Content/site.css"
"~/content/assets/libs/jqueryui/ui-lightness/jquery-ui-1.10.4.custom.min.css",
"~/content/assets/libs/bootstrap/css/bootstrap.min.css",
"~/content/assets/libs/font-awesome/css/font-awesome.min.css",
"~/content/assets/libs/fontello/css/fontello.css",
"~/content/assets/libs/animate-css/animate.min.css",
"~/content/assets/libs/nifty-modal/css/component.css",
"~/content/assets/libs/magnific-popup/magnific-popup.css",
"~/content/assets/libs/ios7-switch/ios7-switch.css",
"~/content/assets/libs/pace/pace.css",
"~/content/assets/libs/sortable/sortable-theme-bootstrap.css",
"~/content/assets/libs/bootstrap-datepicker/css/datepicker.css",
"~/content/assets/libs/prettify/github.css",
"~/content/assets/libs/rickshaw/rickshaw.min.css",
"~/content/assets/libs/morrischart/morris.css",
"~/content/assets/libs/jquery-jvectormap/css/jquery-jvectormap-1.2.2.css",
"~/content/assets/libs/jquery-clock/clock.css",
"~/content/assets/libs/bootstrap-calendar/css/bic_calendar.css",
"~/content/assets/libs/sortable/sortable-theme-bootstrap.css",
"~/content/assets/libs/jquery-weather/simpleweather.css",
"~/content/assets/libs/bootstrap-xeditable/css/bootstrap-editable.css",
"~/content/assets/css/style.css",
"~/content/assets/css/style-responsive.css"
));

BundleTable.EnableOptimizations = true;
}
}
}

 

 

 

 

help me....Thanks in advance........